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KMID : 0603820090150030229
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Journal of Experimental & Biomedical Science
2009 Volume.15 No. 3 p.229 ~ p.232
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Evaluation of DNA Extraction Methods from Low Copy Number (LCN) DNA Samples for Forensic DNA Typing
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Eom Yong-Bin
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Abstract
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DNA isolation for PCR-based short tandem repeat (STR) analysis is essential to recover high yields of amplifiable DNA from low copy number (LCN) DNA samples. There are different methods developed for DNA extraction from the small bloodstain and gloves, commonly found at crime scenes. In order to obtain STR profiles from LCN DNA samples, DNA extraction protocols, namely the automated iPrep™ ChargeSwitch® method, the automated QIAcube™ method, the automated Maxwell® 16 DNA IQ™ Resin method, and the manual QIAamp® DNA Micro Kit method, were evaluated. Extracted DNA was quantified by the QuantifilerTM Human DNA Quantification Kit and DNA profiled by AmpFlSTR® Identifiler® Kit. Results were compared based on the amount of DNA obtained and the completeness of the STR profiles produced. The automated iPrep™ ChargeSwitch® and QIAcube™ methods produced reproducible DNA of sufficient quantity and quality from the dried blood spot. This two automated methods showed a quantity and quality comparable to those of the forensic manual standard protocols normally used in our laboratory. In our hands, the automated DNA extraction method is another obvious choice when the forensic case sample available is bloodstain. The findings of this study indicate that the manual simple modified QIAamp® DNA Micro Kit method is best method to recover high yields of amplifiable DNA from the numerous potential sources of LCN DNA samples.
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KEYWORD
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DNA extraction, low copy number (LCN) DNA, short tandem repeat (STR) analysis
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